MTOR monoclonal antibody (M01), clone 2C5
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More Files
- More Functions
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MTOR is approximately 3ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between AKT1 and MTOR. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-MTOR mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant FRAP1.
Immunogen
MTOR (NP_004949, 1521 a.a. ~ 1620 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
WGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWW
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MTOR is approximately 3ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between AKT1 and MTOR. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-MTOR mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — MTOR
Entrez GeneID
2475GeneBank Accession#
NM_004958Protein Accession#
NP_004949Gene Name
MTOR
Gene Alias
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Gene Description
mechanistic target of rapamycin
Omim ID
601231Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq
Other Designations
FK506 binding protein 12-rapamycin associated protein 1|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1
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Interactome
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Pathway
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Disease
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