FOXM1 monoclonal antibody (M02), clone 5G10
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant FOXM1.
Immunogen
FOXM1 (NP_973731.1, 702 a.a. ~ 801 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LQSAPPLESPQRLLSSEPLDLISVPFGNSSPSDIDVPKPGSPEPQVSGLAANRSLTEGLVLDTMNDSLSKILLDISFPGLDEDPLGPDNINWSQFIPELQ
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
FOXM1 monoclonal antibody (M02), clone 5G10. Western Blot analysis of FOXM1 expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Cell lysate)
FOXM1 monoclonal antibody (M02), clone 5G10 Western Blot analysis of FOXM1 expression in PC-12 ( Cat # L012V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FOXM1 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to FOXM1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — FOXM1
Entrez GeneID
2305GeneBank Accession#
NM_202002Protein Accession#
NP_973731.1Gene Name
FOXM1
Gene Alias
FKHL16, FOXM1B, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TGT3, TRIDENT
Gene Description
forkhead box M1
Omim ID
602341Gene Ontology
HyperlinkGene Summary
drosophila
Other Designations
Forkhead, drosophila, homolog-like 16|M-phase phosphoprotein 2|forkhead-like 16
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Interactome
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Disease
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Publication Reference
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Depletion of intracellular Ca2+ induces FOXM1 SUMOylation and accumulation on the inner nuclear membrane and accelerates G2/M cell cycle transition.
Tzu-Chien Lin, Ping-Jung Chung, Chen-An Shen, Thi My Hang Nguyen, Yi-Syuan Lin, Shih-Chieh Lin, Shih-Chuan Hsiao, Wen-Tai Chiu.
European Journal of Cell Biology 2023 Jun; 102(2):151332.
Application:IF, Human, BG-1 cell.
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Constitutive Phosphorylation Mutation in Fas-associated Death Domain (FADD) Results in Early Cell Cycle Defects.
Stephanie L Osborn, Sue J Sohn, Astar Winoto.
The Journal of Biological Chemistry 2007 Aug; 282(31):22786.
Application:WB-Ce, WB-Ti, Mouse, Mouse T cells, Mouse thymus.
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Depletion of intracellular Ca2+ induces FOXM1 SUMOylation and accumulation on the inner nuclear membrane and accelerates G2/M cell cycle transition.
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