FGF12 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
FGF12 transfected lysate.
Western Blot
Lane 1: FGF12 transfected lysate ( 27.40 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-FGF12 full-length
Host
Human
Theoretical MW (kDa)
27.4
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FGF12 antibody (H00002257-D01P) by Western Blots.
SDS-PAGE Gel
FGF12 transfected lysate.
Western Blot
Lane 1: FGF12 transfected lysate ( 27.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — FGF12
Entrez GeneID
2257GeneBank Accession#
NM_021032.2Protein Accession#
NP_066360.1Gene Name
FGF12
Gene Alias
FGF12B, FHF1
Gene Description
fibroblast growth factor 12
Omim ID
601513Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
fibroblast growth factor 12B|fibroblast growth factor FGF-12b|fibroblast growth factor homologous factor 1|myocyte-activating factor
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