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Last updated: 2016/12/4
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FCER1A monoclonal antibody (M01), clone 2C12-3B6

  • Catalog # : H00002205-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full length recombinant FCER1A.
  • Immunogen:
  • FCER1A (AAH05912.1, 1 a.a. ~ 257 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • MAPAMESPTLLCVALLFFAPDGVLAVPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSLSEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDVYKVIYYKDGEALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPREKYWLQFFIPLLVVILFAVDTGLFISTQQQVTFLLKIKRTRKGFRLLNPHPKPNPKNN
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00002205-M01
    Western Blot detection against Immunogen (54.01 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • FCER1A monoclonal antibody (M01), clone 2C12-3B6 Western Blot analysis of FCER1A expression in A-549 ( Cat # L025V1 ).
  • PDF DownloadProtocol Download
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody (M01), clone 2C12-3B6.

    Lane 1: FCER1A transfected lysate(29.6 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged FCER1A is 1 ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of FCER1A over-expressed 293 cell line, cotransfected with FCER1A Validated Chimera RNAi ( Cat # H00002205-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FCER1A monoclonal antibody (M01), clone 2C12-3B6 (Cat # H00002205-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2205
  • Gene Name:
  • FCER1A
  • Gene Alias:
  • FCE1A,FcERI
  • Gene Description:
  • Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
  • Gene Summary:
  • The IgE receptor plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more high-affinity IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. The IgE receptor consists of 3 subunits: alpha, beta (MIM 147138), and gamma (MIM 147139); only the alpha subunit is glycosylated.[supplied by OMIM
  • Other Designations:
  • Fc IgE receptor, alpha polypeptide,Fc epsilon RI alpha-chain,Fc-epsilon RI-alpha,high affinity immunoglobulin epsilon receptor alpha-subunit,immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide
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