FASN monoclonal antibody (M01), clone 3F2-1F3
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant FASN.
Immunogen
FASN (AAH07909, 1 a.a. ~ 439 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLSSFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAAVNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASGLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (74.03 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
FASN monoclonal antibody (M01), clone 3F2-1F3 Western Blot analysis of FASN expression in 293 ( Cat # L026V1 ).Western Blot (Transfected lysate)
Western Blot analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody (M01), clone 3F2-1F3.
Lane 1: FASN transfected lysate(48.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to FASN on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 10 ug/ml]Immunoprecipitation
Immunoprecipitation of FASN transfected lysate using anti-FASN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FASN MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FASN is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to FASN on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — FASN
Entrez GeneID
2194GeneBank Accession#
BC007909Protein Accession#
AAH07909Gene Name
FASN
Gene Alias
FAS, MGC14367, MGC15706, OA-519, SDR27X1
Gene Description
fatty acid synthase
Omim ID
600212Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq
Other Designations
short chain dehydrogenase/reductase family 27X, member 1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Metabolic reprogramming in memory CD4 T cell responses of old adults.
Yanes RE, Zhang H, Shen Y, Weyand CM, Goronzy JJ.
Clinical immunology (Orlando, Fla.) 2019 Oct; 207:58.
Application:IHC, Human, Human memory T cells.
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Myoglobin expression in prostate cancer is correlated to androgen receptor expression and markers of tumor hypoxia.
Meller S, Bicker A, Montani M, Ikenberg K, Rostamzadeh B, Sailer V, Wild P, Dietrich D, Uhl B, Sulser T, Moch H, Gorr TA, Stephan C, Jung K, Hankeln T, Kristiansen G.
Virchows Archiv 2014 Oct; 465(4):419.
Application:IHC, Human, Carcinoma, prostate cancer tissue.
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Old proteins - new locations: myoglobin, haemoglobin, neuroglobin and cytoglobin in solid tumours and cancer cells.
Gorr TA, Wichmann D, Pilarsky C, Theurillat JP, Fabrizius A, Laufs T, Bauer T, Koslowski M, Horn S, Burmester T, Hankeln T, Kristiansen G.
Acta Physiologica 2011 Jul; 202(3):563.
Application:IHC, Human, Breast cancer.
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Endogenous myoglobin in human breast cancer is a hallmark of luminal cancer phenotype.
Kristiansen G, Rose M, Geisler C, Fritzsche FR, Gerhardt J, Luke C, Ladhoff AM, Knuchel R, Dietel M, Moch H, Varga Z, Theurillat JP, Gorr TA, Dahl E.
British Journal of Cancer 2010 Jun; 102(12):1736.
Application:IF, IHC, Human, Human breast cancer tissues.
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Metabolic reprogramming in memory CD4 T cell responses of old adults.
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