FAP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
FAP transfected lysate.
Western Blot
Lane 1: FAP transfected lysate ( 87.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-FAP full-length
Host
Human
Theoretical MW (kDa)
87.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FAP antibody (H00002191-B01) by Western Blots.
SDS-PAGE Gel
FAP transfected lysate.
Western Blot
Lane 1: FAP transfected lysate ( 87.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — FAP
Entrez GeneID
2191GeneBank Accession#
NM_004460.2Protein Accession#
-Gene Name
FAP
Gene Alias
DKFZp686G13158, DPPIV, FAPA
Gene Description
fibroblast activation protein, alpha
Omim ID
600403Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. [provided by RefSeq
Other Designations
fibroblast activation protein, alpha subunit|integral membrane serine protease|seprase
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Interactome
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Disease
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Publication Reference
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RIPK3-MLKL-Mediated Neutrophil Death Requires Concurrent Activation of Fibroblast Activation Protein-α.
Xiaoliang Wang, Francois Gessier, Remo Perozzo, Darko Stojkov, Aref Hosseini, Keyvan Amirshahrokhi, Stefan Kuchen, Shida Yousefi, Pius Lötscher, Hans-Uwe Simon.
Journal of immunology 2020 Sep; 205(6):1653.
Application：WB, Human, HEK 293T cells.
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RIPK3-MLKL-Mediated Neutrophil Death Requires Concurrent Activation of Fibroblast Activation Protein-α.
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