F12 293T Cell Transient Overexpression Lysate(Denatured)
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Specifications
Transfected Cell Line
293T
Plasmid
pCMV-F12 full-length
Host
Human
Theoretical MW (kDa)
33.11
Interspecies Antigen Sequence
Mouse (73); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-F12 antibody (H00002161-B01) by Western Blots.
SDS-PAGE Gel
F12 transfected lysate.
Western Blot
Lane 1: F12 transfected lysate ( 33.11 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — F12
Entrez GeneID
2161GeneBank Accession#
BC012390.1Protein Accession#
AAH12390.1Gene Name
F12
Gene Alias
HAE3, HAEX, HAF
Gene Description
coagulation factor XII (Hageman factor)
Gene Ontology
HyperlinkGene Summary
This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq
Other Designations
Hageman factor|coagulation factor XII
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Interactomes
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Pathways
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Diseases
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