F9 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-F9 full-length
Host
Human
Theoretical MW (kDa)
51.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-F9 antibody (H00002158-B01) by Western Blots.
SDS-PAGE Gel
F9 transfected lysate.
Western Blot
Lane 1: F9 transfected lysate ( 51.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — F9
Entrez GeneID
2158GeneBank Accession#
NM_000133.2Protein Accession#
-Gene Name
F9
Gene Alias
FIX, HEMB, MGC129641, MGC129642, PTC
Gene Description
coagulation factor IX
Omim ID
306900Gene Ontology
HyperlinkGene Summary
This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. [provided by RefSeq
Other Designations
Christmas factor|OTTHUMP00000024154|coagulant factor IX|factor 9|factor IX|plasma thromboplastic component
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Interactome
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Pathway
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Disease
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