EWSR1 monoclonal antibody (M01), clone 5C10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of EWSR1 expression in transfected 293T cell line by EWSR1 monoclonal antibody (M01), clone 5C10.
Lane 1: EWSR1 transfected lysate(68.5 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to EWSR1 on formalin-fixed paraffin-embedded human urinary bladder. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EWSR1 is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.3 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant EWSR1.
Immunogen
EWSR1 (NP_005234, 358 a.a. ~ 453 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SDNSAIYVQGLNDSVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNS
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.3 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of EWSR1 expression in transfected 293T cell line by EWSR1 monoclonal antibody (M01), clone 5C10.
Lane 1: EWSR1 transfected lysate(68.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to EWSR1 on formalin-fixed paraffin-embedded human urinary bladder. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EWSR1 is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — EWSR1
Entrez GeneID
2130GeneBank Accession#
NM_005243Protein Accession#
NP_005234Gene Name
EWSR1
Gene Alias
EWS
Gene Description
Ewing sarcoma breakpoint region 1
Omim ID
133450Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq
Other Designations
Ewings sarcoma EWS-Fli1 (type 1) oncogene|bK984G1.4 (Ewing sarcoma breakpoint region 1 protein)
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