EPAS1 monoclonal antibody (M08), clone 1G2
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant EPAS1.
Immunogen
EPAS1 (NP_001421.2, 28 a.a. ~ 347 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVKVYNNCPPHNSLCGYKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELIGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEI
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (60.83 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of EPAS1 expression in transfected 293T cell line by EPAS1 monoclonal antibody (M08), clone 1G2.
Lane 1: EPAS1 transfected lysate (Predicted MW: 96.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EPAS1 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to EPAS1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — EPAS1
Entrez GeneID
2034GeneBank Accession#
NM_001430.3Protein Accession#
NP_001421.2Gene Name
EPAS1
Gene Alias
ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73
Gene Description
endothelial PAS domain protein 1
Omim ID
603349Gene Ontology
HyperlinkOther Designations
HIF-1alpha-like factor
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Interactome
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Pathway
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Disease
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