EN2 monoclonal antibody (M03), clone 1E1
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More Files
- More Functions
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EN2 is 3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to EN2 on HeLa cell . [antibody concentration 40 ug/ml]
Western Blot detection against Immunogen (39.38 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant EN2.
Immunogen
EN2 (NP_001418.2, 86 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GTCCAGAGGGRGGGAGGEGGASGAEGGGGAGGSEQLLGSGSREPRQNPPCAPGAGGPLPAAGSDSPGDGEGGSKTLSLHGGAKKGGDPGGPLDGSLKARGLGGGDLSVSSDSDSSQAGANLGAQP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (79); Rat (78)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (39.38 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EN2 is 3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to EN2 on HeLa cell . [antibody concentration 40 ug/ml] -
Gene Info — EN2
Entrez GeneID
2020GeneBank Accession#
NM_001427Protein Accession#
NP_001418.2Gene Name
EN2
Gene Alias
AUTS1, AUTS10
Gene Description
engrailed homeobox 2
Gene Ontology
HyperlinkGene Summary
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq
Other Designations
engrailed homolog 2|engrailed-2
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Interactome
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Disease
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Publication Reference
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Aptamer-antibody hybrid ELONA that uses hybridization chain reaction to detect a urinary biomarker EN2 for bladder and prostate cancer.
Eunseon Kim, Minji Kang, Changill Ban.
Scientific Reports 2022 Jul; 12(1):11523.
Application：Aptamer-antibody hybrid (ELONA), Human, Urine.
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Aptamer-antibody hybrid ELONA that uses hybridization chain reaction to detect a urinary biomarker EN2 for bladder and prostate cancer.
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