ELA1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ELA1 full-length
Host
Human
Theoretical MW (kDa)
27.8
Interspecies Antigen Sequence
Mouse (85); Rat (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ELA1 antibody (H00001990-B01) by Western Blots.
SDS-PAGE Gel
ELA1 transfected lysate.
Western Blot
Lane 1: ELA1 transfected lysate ( 27.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ELA1
Entrez GeneID
1990GeneBank Accession#
NM_001971Protein Accession#
NP_001962Gene Name
ELA1
Gene Alias
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Gene Description
elastase 1, pancreatic
Omim ID
130120Gene Ontology
HyperlinkGene Summary
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq
Other Designations
pancreatic elastase I
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Interactome
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Disease
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