EIF5A monoclonal antibody (M01), clone 8C1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of EIF5A expression in transfected 293T cell line by EIF5A monoclonal antibody (M01), clone 8C1.
Lane 1: EIF5A transfected lysate(16.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EIF5A is 3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to EIF5A on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (43.2 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant EIF5A.
Immunogen
EIF5A (NP_001961.1, 1 a.a. ~ 154 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (43.2 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of EIF5A expression in transfected 293T cell line by EIF5A monoclonal antibody (M01), clone 8C1.
Lane 1: EIF5A transfected lysate(16.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EIF5A is 3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to EIF5A on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — EIF5A
Entrez GeneID
1984GeneBank Accession#
NM_001970.3Protein Accession#
NP_001961.1Gene Name
EIF5A
Gene Alias
EIF-5A, EIF5A1, MGC104255, MGC99547, eIF5AI
Gene Description
eukaryotic translation initiation factor 5A
Omim ID
600187Gene Ontology
HyperlinkOther Designations
OTTHUMP00000128384|uORF A
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