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Last updated: 2016/11/27
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EIF4E 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001977-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-EIF4E full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 23.98
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-EIF4E antibody (H00001977-B01) by Western Blots.
    Western Blot
    QC Testing of H00001977-T01
    Lane 1: EIF4E transfected lysate ( 25.1 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00001977-T01
    EIF4E transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1977
  • Gene Name:
  • EIF4E
  • Gene Alias:
  • CBP,EIF4E1,EIF4EL1,EIF4F,MGC111573
  • Gene Description:
  • eukaryotic translation initiation factor 4E
  • Gene Summary:
  • All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM
  • Other Designations:
  • eIF-4F 25 kDa subunit,eukaryotic translation initiation factor 4E-like 1,mRNA cap-binding protein
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