EIF4B (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human EIF4B full-length ORF ( AAH98437.1, 1 a.a. - 611 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGDVSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPCRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSASTSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRPRERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDGGNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDGEDENEGEDYAE
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
95.5
Interspecies Antigen Sequence
Mouse (95); Rat (96)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — EIF4B
Entrez GeneID
1975GeneBank Accession#
BC098437.1Protein Accession#
AAH98437.1Gene Name
EIF4B
Gene Alias
EIF-4B, PRO1843
Gene Description
eukaryotic translation initiation factor 4B
Omim ID
603928Gene Ontology
HyperlinkOther Designations
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Interactome
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Pathway
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Publication Reference
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Mitotic MELK-eIF4B signaling controls protein synthesis and tumor cell survival.
Wang Y, Begley M, Li Q,Huang HT, Lako A, Eck MJ, Gray NS, Mitchisond TJ, Cantley LC, Zhao JJ.
PNAS 2016 Aug; 113(35):9810.
Application:Incubated, Recombinant protein.
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The Pim-1 Protein Kinase Is an Important Regulator of MET Receptor Tyrosine Kinase Levels and Signaling.
Cen B, Xiong Y, Song JH, Mahajan S, DuPont R, McEachern K, DeAngelo DJ, Cortes JE, Minden MD, Ebens A, Mims A, LaRue AC, Kraft AS.
Molecular and Cellular Biology 2014 Jul; 34(13):2517.
Application:WB-Ce, WB-Tr, Human, U2OS, HeLa, BPH1, PC3-LN4, 293T, MKN45, H1993 cells.
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Proteomic Identification of Hsp70 as a new Plk1 Substrate in Arsenic Trioxide-Induced Mitotically Arrested Cells.
Chen Y, Lin YP, Chow LP, Lee TC.
Proteomics 2011 Nov; 11(22):4331.
Application:KA, WB-Re, Recombinant proteins.
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Mitotic MELK-eIF4B signaling controls protein synthesis and tumor cell survival.
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