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EIF2S1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001965-T03
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-EIF2S1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 36.1
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-EIF2S1 antibody (H00001965-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001965-T03
    EIF2S1 transfected lysate.
    Western Blot
    QC Testing of H00001965-T03
    Lane 1: EIF2S1 transfected lysate ( 36.10 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1965
  • Gene Name:
  • EIF2S1
  • Gene Alias:
  • EIF-2,EIF-2A,EIF-2alpha,EIF2,EIF2A
  • Gene Description:
  • eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • Gene Summary:
  • The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM
  • Other Designations:
  • eIF-2-alpha,eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD )
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