EEF1G monoclonal antibody (M03), clone S1
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More Files
- More Functions
Immunoprecipitation
Immunoprecipitation of EEF1G transfected lysate using anti-EEF1G monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EEF1G MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EEF1G is 0.1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to EEF1G on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (73.59 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant EEF1G.
Immunogen
EEF1G (AAH15813.1, 1 a.a. ~ 437 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAGTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHFGQTNRTPEFLRKFPAGKVPAFEGDDGFCVFESNAIAYHVSNEELRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHDKQATENAKEEVRRILGLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWRKLDPGSEETQTLVREYFSWEGAFQHVGKAFNQGKIFK
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (73.59 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of EEF1G transfected lysate using anti-EEF1G monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EEF1G MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EEF1G is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to EEF1G on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — EEF1G
Entrez GeneID
1937GeneBank Accession#
BC015813Protein Accession#
AAH15813.1Gene Name
EEF1G
Gene Alias
EF1G, GIG35
Gene Description
eukaryotic translation elongation factor 1 gamma
Omim ID
130593Gene Ontology
HyperlinkGene Summary
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq
Other Designations
EF-1-gamma|PRO1608|eEF-1B gamma|elongation factor 1-gamma|pancreatic tumor-related protein|translation elongation factor eEF-1 gamma chain
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