EDA (Human) Recombinant Protein
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More Files
- More Functions
SDS-PAGE Gel
Western Blot
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Specification
Product Description
Purified EDA (NP_001390.1 245 a.a. - 391 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
Transfected Cell Line
Human HEK293T cells
Sequence
ENQPAVVHLQGQGSAIQVKNDLSGGVLNDWSRITMNPKVFKLHPRSGELEVLVDGTYFIYSQVEVYYINFTDFASYEVVVDEKPFLQCTRSIETGKTNYNTCYTAGVCLLKARQKIAVKMVHADISINMSKHTTFFGAIRLGEAPAS
Host
Human
Theoretical MW (kDa)
21.45
Form
Liquid
Preparation Method
Transfection of pSuper-EDA plasmid into HEK293T cell, and the expressed protein was purified by Strep-Tactin affinity column.
Purification
Strep-Tactin affinity columns
Concentration
≥ 10 ug/ml
Quality Control Testing
SDS-PAGE and Western Blot
SDS-PAGE Gel
Western Blot
Storage Buffer
100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
Protein Interaction
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Gene Info — EDA
Entrez GeneID
1896GeneBank Accession#
NM_001399.1Protein Accession#
NP_001390.1Gene Name
EDA
Gene Alias
ED1, ED1-A1, ED1-A2, EDA1, EDA2, HED, XHED, XLHED
Gene Description
ectodysplasin A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000023461|OTTHUMP00000023463|X-linked anhidroitic ectodermal dysplasia protein|ectodermal dysplasia 1, anhidrotic|ectodermal dysplasia, anhidrotic (hypohydrotic)|ectodysplasin-A
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Interactome
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Pathway
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