ECH1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
Western Blot
Lane 1: ECH1 transfected lysate ( 35.8 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
ECH1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ECH1 full-length
Host
Human
Theoretical MW (kDa)
36.19
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ECH1 antibody (H00001891-B01) by Western Blots.
Western Blot
Lane 1: ECH1 transfected lysate ( 35.8 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
ECH1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ECH1
Entrez GeneID
1891GeneBank Accession#
NM_001398.2Protein Accession#
-Gene Name
ECH1
Gene Alias
HPXEL
Gene Description
enoyl Coenzyme A hydratase 1, peroxisomal
Omim ID
600696Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq
Other Designations
delta3,5-delta2,4-dienoyl-CoA isomerase|dienoyl-CoA isomerase|peroxisomal enoyl-CoA hydratase 1|peroxisomal enoyl-coenzyme A hydratase-like protein
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Interactome
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