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ECH1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001891-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ECH1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 36.19
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ECH1 antibody (H00001891-B01) by Western Blots.
    Western Blot
    QC Testing of H00001891-T01
    Lane 1: ECH1 transfected lysate ( 35.8 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00001891-T01
    ECH1 transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1891
  • Protein Accession#:
  • -
  • Gene Name:
  • ECH1
  • Gene Alias:
  • HPXEL
  • Gene Description:
  • enoyl Coenzyme A hydratase 1, peroxisomal
  • Gene Summary:
  • This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq
  • Other Designations:
  • delta3,5-delta2,4-dienoyl-CoA isomerase,dienoyl-CoA isomerase,peroxisomal enoyl-CoA hydratase 1,peroxisomal enoyl-coenzyme A hydratase-like protein
  • Gene Pathway
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