EBF1 monoclonal antibody (M02), clone 1G8
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant EBF1.
Immunogen
EBF1 (AAH38805, 1 a.a. ~ 591 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFSKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQLPALANTSVHAGMMGVNSFSGQLAVNVSEASQATNQGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNGYGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAPVVRPQTSPPPTCTSTNGNSLQAISGMIVPPM
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (90.75 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of EBF1 expression in transfected 293T cell line by EBF1 monoclonal antibody (M02), clone 1G8.
Lane 1: EBF1 transfected lysate(64 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EBF1 is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of EBF1 over-expressed 293 cell line, cotransfected with EBF1 Validated Chimera RNAi ( Cat # H00001879-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EBF1 monoclonal antibody (M02), clone 1G8 (Cat # H00001879-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — EBF1
Entrez GeneID
1879GeneBank Accession#
BC038805Protein Accession#
AAH38805Gene Name
EBF1
Gene Alias
COE1, EBF, FLJ39389, FLJ41763, O/E-1, OLF1
Gene Description
early B-cell factor 1
Omim ID
164343Gene Ontology
HyperlinkGene Summary
Olf and EBF transcription factor 1|early B-cell factor|olfactory neuronal transcription factor 1
Other Designations
Collier, Olf and EBF transcription factor 1|early B-cell factor|olfactory neuronal transcription factor 1
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Interactome
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Disease
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Publication Reference
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Amelioration of experimental autoimmune encephalomyelitis by in vivo reprogramming of macrophages using pro-resolving factors.
Thierry Gauthier, Omayra Martin-Rodriguez, Cécile Chagué, Anna Daoui, Adam Ceroi, Alexis Varin, Francis Bonnefoy, Séverine Valmary-Degano, Mélanie Couturier, Susanne Behlke, Philippe Saas, Pierre-François Cartron, Sylvain Perruche.
Journal of Neuroinflammation 2023 Dec; 20(1):307.
Application:ELISA, Mouse, Spleen CD11b+ cells.
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The Blk pathway functions as a tumor suppressor in chronic myeloid leukemia stem cells.
Zhang H, Peng C, Hu Y, Li H, Sheng Z, Chen Y, Sullivan C, Cerny J, Hutchinson L, Higgins A, Miron P, Zhang X, Brehm MA, Li D, Green MR, Li S.
Nature Genetics 2012 Jul; 44(8):861.
Application:ChIP, Mouse, BaF3 cells.
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Amelioration of experimental autoimmune encephalomyelitis by in vivo reprogramming of macrophages using pro-resolving factors.
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