EBF1 monoclonal antibody (M01), clone 1C12
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant EBF1.
Immunogen
EBF1 (AAH38805, 1 a.a. ~ 591 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFSKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQLPALANTSVHAGMMGVNSFSGQLAVNVSEASQATNQGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNGYGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAPVVRPQTSPPPTCTSTNGNSLQAISGMIVPPM
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (90.75 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of EBF1 expression in transfected 293T cell line by EBF1 monoclonal antibody (M01), clone 1C12.
Lane 1: EBF1 transfected lysate(64 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EBF1 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — EBF1
Entrez GeneID
1879GeneBank Accession#
BC038805Protein Accession#
AAH38805Gene Name
EBF1
Gene Alias
COE1, EBF, FLJ39389, FLJ41763, O/E-1, OLF1
Gene Description
early B-cell factor 1
Omim ID
164343Gene Ontology
HyperlinkGene Summary
Olf and EBF transcription factor 1|early B-cell factor|olfactory neuronal transcription factor 1
Other Designations
Collier, Olf and EBF transcription factor 1|early B-cell factor|olfactory neuronal transcription factor 1
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Interactome
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Disease
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Publication Reference
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CEBPA phase separation links transcriptional activity and 3D chromatin hubs.
Marie Christou-Kent, Sergi Cuartero, Carla Garcia-Cabau, Julia Ruehle, Julian Naderi, Julia Erber, Maria Victoria Neguembor, Marcos Plana-Carmona, Marc Alcoverro-Bertran, Luisa De Andres-Aguayo, Antonios Klonizakis, Eric Julià-Vilella, Cian Lynch, Manuel Serrano, Denes Hnisz, Xavier Salvatella, Thomas Graf, Grégoire Stik.
Cell Reports 2023 Aug; 42(8):112897.
Application:IF, Mouse, B cells.
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Early B-cell Factor1 (Ebf1) Promotes Early Osteoblast Differentiation But Suppresses Osteoblast Function.
Vappu Nieminen-Pihala, Kati Tarkkonen, Julius Laine, Petri Rummukainen, Lauri Saastamoinen, Kenici Nagano, Roland Baron, Riku Kiviranta.
Bone 2021 Feb; 146:115884.
Application:ChIP, Mouse, MC3T3-E1 cells.
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De Novo Mutations in EBF3 Cause a Neurodevelopmental Syndrome.
Sleven H, Welsh SJ, Yu J, Churchill ME, Wright CF, Henderson A, Horvath R, Rankin J, Vogt J, Magee A, McConnell V, Green A, King MD, Cox H, Armstrong L, Lehman A, Nelson TN; Deciphering Developmental Disorders study; CAUSES study, Williams J, Clouston P, Hagman J, Németh AH.
American Journal of Human Genetics 2017 Jan; 100(1):138.
Application:WB, Mouse, μM2.21 cells.
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Early B-Cell Factor-1 (Ebf1) is a key regulator of metabolic and inflammatory signaling pathways in mature adipocytes.
Griffin MJ, Zhou Y, Kang S, Zhang X, Mikkelsen TS, Rosen ED.
The Journal of Biological Chemistry 2013 Dec; 288(50):35925.
Application:ChIP, Mouse, 3T3-L1 adipocytes.
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Cellular origin and pathophysiology of chronic lymphocytic leukemia.
Seifert M, Sellmann L, Bloehdorn J, Wein F, Stilgenbauer S, Dürig J, Küppers R.
The Journal of Experimental Medicine 2012 Nov; 209(12):2183.
Application:WB-Ce, Human, CLL, CD5+ B cells.
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CEBPA phase separation links transcriptional activity and 3D chromatin hubs.
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