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E4F1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001877-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-E4F1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 86.35
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-E4F1 antibody (H00001877-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001877-T01
    E4F1 transfected lysate.
    Western Blot
    QC Testing of H00001877-T01
    Lane 1: E4F1 transfected lysate ( 86.35 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1877
  • Gene Name:
  • E4F1
  • Gene Alias:
  • E4F,MGC99614
  • Gene Description:
  • E4F transcription factor 1
  • Gene Summary:
  • The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. [provided by RefSeq
  • Other Designations:
  • p120E4F
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