E4F1 monoclonal antibody (M03), clone 4B10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10.
Lane 1: E4F1 transfected lysate(83.5 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged E4F1 is 0.1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to E4F1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant E4F1.
Immunogen
E4F1 (NP_004415.2, 685 a.a. ~ 784 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10.
Lane 1: E4F1 transfected lysate(83.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged E4F1 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to E4F1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — E4F1
Entrez GeneID
1877GeneBank Accession#
NM_004424Protein Accession#
NP_004415.2Gene Name
E4F1
Gene Alias
E4F, MGC99614
Gene Description
E4F transcription factor 1
Omim ID
603022Gene Ontology
HyperlinkGene Summary
The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. [provided by RefSeq
Other Designations
p120E4F
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Interactome
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Publication Reference
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Vorinostat decrease M2 macrophage polarization through ARID1A6488delG/HDAC6/IL-10 signaling pathway in endometriosis-associated ovarian carcinoma.
Tsung-Hua Hsieh, Chia-Yi Hsu, Chia-Wei Wu, Shih-Ho Wang, Cheng-Hsi Yeh, Kuang-Hung Cheng, Eing-Mei Tsai.
Biomedicine & Pharmacotherapy 2023 May; 161:114500.
Application：WB-Tr, Human, TOV-112D, TOV-21G cells.
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Vorinostat decrease M2 macrophage polarization through ARID1A6488delG/HDAC6/IL-10 signaling pathway in endometriosis-associated ovarian carcinoma.
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