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E2F2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001870-T04
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-E2F2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 47.5
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-E2F2 antibody (H00001870-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001870-T04
    E2F2 transfected lysate.
    Western Blot
    QC Testing of H00001870-T04
    Lane 1: E2F2 transfected lysate ( 47.50 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1870
  • Gene Name:
  • E2F2
  • Gene Alias:
  • E2F-2
  • Gene Description:
  • E2F transcription factor 2
  • Gene Summary:
  • The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000003257
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