E2F2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
E2F2 transfected lysate.
Western Blot
Lane 1: E2F2 transfected lysate ( 9.24 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-E2F2 full-length
Host
Human
Theoretical MW (kDa)
9.24
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-E2F2 antibody (H00001870-B01) by Western Blots.
SDS-PAGE Gel
E2F2 transfected lysate.
Western Blot
Lane 1: E2F2 transfected lysate ( 9.24 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — E2F2
Entrez GeneID
1870GeneBank Accession#
BC007609Protein Accession#
AAH07609Gene Name
E2F2
Gene Alias
E2F-2
Gene Description
E2F transcription factor 2
Omim ID
600426Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq
Other Designations
OTTHUMP00000003257
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Interactome
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Pathway
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Disease
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