DUT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DUT transfected lysate.
Western Blot
Lane 1: DUT transfected lysate ( 26.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DUT full-length
Host
Human
Theoretical MW (kDa)
26.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DUT antibody (H00001854-B01) by Western Blots.
SDS-PAGE Gel
DUT transfected lysate.
Western Blot
Lane 1: DUT transfected lysate ( 26.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DUT
Entrez GeneID
1854GeneBank Accession#
NM_001025248Protein Accession#
NP_001020419Gene Name
DUT
Gene Alias
FLJ20622, dUTPase
Gene Description
deoxyuridine triphosphatase
Omim ID
601266Gene Ontology
HyperlinkGene Summary
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq
Other Designations
dUTP nucleotidohydrolase|dUTP pyrophosphatase|deoxyuridine 5'-triphosphate nucleotidohydrolase
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Interactome
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Pathway
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Disease
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