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Last updated: 2017/12/10

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DUT 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001854-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-DUT full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 26.6
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DUT antibody (H00001854-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001854-T01
    DUT transfected lysate.
    Western Blot
    QC Testing of H00001854-T01
    Lane 1: DUT transfected lysate ( 26.6 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1854
  • Gene Name:
  • DUT
  • Gene Alias:
  • FLJ20622,dUTPase
  • Gene Description:
  • deoxyuridine triphosphatase
  • Gene Summary:
  • This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq
  • Other Designations:
  • dUTP nucleotidohydrolase,dUTP pyrophosphatase,deoxyuridine 5'-triphosphate nucleotidohydrolase
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