DUSP6 monoclonal antibody (M01), clone 3G2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody (M01), clone 3G2.
Lane 1: DUSP6 transfected lysate(42 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DUSP6 is approximately 1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi ( Cat # H00001848-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody (M01), clone 3G2 (Cat # H00001848-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to DUSP6 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (67.65 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant DUSP6.
Immunogen
DUSP6 (AAH03143, 1 a.a. ~ 381 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MIDTLRPVPFASEMAISKTVAWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGNLPVRALFTRGEDRDRFTRRCGTDTVVLYDESSSDWNENTGGESLLGLLLKKLKDEGCRAFYLEGGFSKFQAEFSLHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEILPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (67.65 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody (M01), clone 3G2.
Lane 1: DUSP6 transfected lysate(42 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DUSP6 is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi ( Cat # H00001848-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody (M01), clone 3G2 (Cat # H00001848-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to DUSP6 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — DUSP6
Entrez GeneID
1848GeneBank Accession#
BC003143Protein Accession#
AAH03143Gene Name
DUSP6
Gene Alias
MKP3, PYST1
Gene Description
dual specificity phosphatase 6
Omim ID
602748Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
MAP kinase phosphatase 3|serine/threonine specific protein phosphatase
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Interactome
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Pathway
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Disease
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Publication Reference
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TSH Signaling Overcomes B-RafV600E–Induced Senescence in Papillary Thyroid Carcinogenesis through Regulation of DUSP6.
Kim YH, Choi YW, Han JH, Lee J, Soh EY, Park SH, Kim JH, Park TJ.
Neoplasia 2014 Dec; 16(12):1107.
Application：IHC, WB-Tr, Human, Papillary thyroid carcinoma, Thyrocytes, SNU790 cells.
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Dual specificity phosphatase 6 as a predictor of invasiveness in papillary thyroid cancer.
Lee JU, Huang S, Lee MH, Lee SE, Ryu MJ, Kim SJ, Kim YK, Kim SY, Joung KH, Kim JM, Shong M, Jo YS.
European Journal of Endocrinology 2012 Apr; 167(1):93.
Application：IHC-P, WB, Human, FRO cells, Human papillary thyroid cancer.
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Down-Regulation of DUSP6 Expression in Lung Cancer: Its Mechanism and Potential Role in Carcinogenesis.
Okudela K, Yazawa T, Woo T, Sakaeda M, Ishii J, Mitsui H, Shimoyamada H, Sato H, Tajiri M, Ogawa N, Masuda M, Takahashi T, Sugimura H, Kitamura H.
The American Journal of Pathology 2009 Aug; 175(2):867.
Application：WB, IHC, Human, 16HBE14o, A549, H358, H1299 cells, Lung.
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TSH Signaling Overcomes B-RafV600E–Induced Senescence in Papillary Thyroid Carcinogenesis through Regulation of DUSP6.
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