DPH1 rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human DPH1 peptide using ARM Technology.
Immunogen
A synthetic peptide of human DPH1 is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human DPH1 peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — DPH1
Entrez GeneID
1801GeneBank Accession#
DPH1Gene Name
DPH1
Gene Alias
DPH2L, DPH2L1, FLJ33211, OVCA1
Gene Description
DPH1 homolog (S. cerevisiae)
Omim ID
603527Gene Ontology
HyperlinkGene Summary
Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM
Other Designations
DPH-like 1|DPH2-like 1|candidate tumor suppressor in ovarian cancer 1|diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces)-like 1|diptheria toxin resistance protein required for diphthamide biosynthesis-like 1|ovarian c
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Interactome
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