DNTT monoclonal antibody (M01), clone 4H5

Catalog # H00001791-M01

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Size:100 ug
Price: USD $ 335.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western Blot analysis of DNTT expression in transfected 293T cell line by DNTT monoclonal antibody (M01), clone 4H5.

Lane 1: DNTT transfected lysate (Predicted MW: 58.4 KDa).
Lane 2: Non-transfected lysate.

Sandwich ELISA (Recombinant protein)
Application

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged DNTT is approximately 0.1ng/ml as a capture antibody.

QC Test

Western Blot detection against Immunogen (37.51 KDa) .

  • Specification

    Product Description

    Mouse monoclonal antibody raised against a partial recombinant DNTT.

    Immunogen

    DNTT (AAH12920, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Sequence

    MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKGFRVENELSDSVTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIEC

    Host

    Mouse

    Reactivity

    Human

    Interspecies Antigen Sequence

    Mouse (81); Rat (83)

    Isotype

    IgG1 Kappa

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Western Blot detection against Immunogen (37.51 KDa) .

    Storage Buffer

    In 1x PBS, pH 7.4

    Storage Instruction

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot (Transfected lysate)

    Western Blot analysis of DNTT expression in transfected 293T cell line by DNTT monoclonal antibody (M01), clone 4H5.

    Lane 1: DNTT transfected lysate (Predicted MW: 58.4 KDa).
    Lane 2: Non-transfected lysate.

    Western Blot (Recombinant protein)

    Sandwich ELISA (Recombinant protein)

    Detection limit for recombinant GST tagged DNTT is approximately 0.1ng/ml as a capture antibody.

    ELISA

  • Gene Info — DNTT

    Entrez GeneID

    1791

    GeneBank Accession#

    BC012920

    Protein Accession#

    AAH12920

    Gene Name

    DNTT

    Gene Alias

    TDT

    Gene Description

    deoxynucleotidyltransferase, terminal

    Omim ID

    187410

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq

    Other Designations

    DNA nucleotidylexotransferase|OTTHUMP00000020171|nucleosidetriphosphate:DNA deoxynucleotidylexotransferase|terminal addition enzyme|terminal deoxynucleotidyltransferase|terminal deoxyribonucleotidyltransferase|terminal transferase

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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