DLAT purified MaxPab mouse polyclonal antibody (B01P)
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More Files
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Western Blot (Tissue lysate)
DLAT MaxPab polyclonal antibody. Western Blot analysis of DLAT expression in rat brain.
Western Blot (Cell lysate)
DLAT MaxPab polyclonal antibody. Western Blot analysis of DLAT expression in HepG2.
Western Blot (Transfected lysate)
Western Blot analysis of DLAT expression in transfected 293T cell line (H00001737-T01) by DLAT MaxPab polyclonal antibody.
Lane 1: DLAT transfected lysate(71.17 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to DLAT on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human DLAT protein.
Immunogen
DLAT (NP_001922.2, 1 a.a. ~ 647 a.a) full-length human protein.
Sequence
MWRVCARRAQNVAPWAGLEARWTALQEVPGTPRVTSRSGPAPARRNSVTTGYGGVRALCGWTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPTPPPVAAVPPTPQPLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDIDSFVPSKVAPAPAAVVPPTGPGMAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSIDVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRKYLEKPITMLL
Host
Mouse
Reactivity
Human, Rat
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
DLAT MaxPab polyclonal antibody. Western Blot analysis of DLAT expression in rat brain.Western Blot (Cell lysate)
DLAT MaxPab polyclonal antibody. Western Blot analysis of DLAT expression in HepG2.Western Blot (Transfected lysate)
Western Blot analysis of DLAT expression in transfected 293T cell line (H00001737-T01) by DLAT MaxPab polyclonal antibody.
Lane 1: DLAT transfected lysate(71.17 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to DLAT on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — DLAT
Entrez GeneID
1737GeneBank Accession#
NM_001931.2Protein Accession#
NP_001922.2Gene Name
DLAT
Gene Alias
DLTA, PDC-E2, PDCE2
Gene Description
dihydrolipoamide S-acetyltransferase
Omim ID
608770Gene Ontology
HyperlinkGene Summary
The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM
Other Designations
70 kDa mitochondrial autoantigen of primary biliary cirrhosis|E2 component of pyruvate dehydrogenase complex|M2 antigen complex 70 kDa subunit
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
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