DIAPH1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DIAPH1 full-length
Host
Human
Theoretical MW (kDa)
44.44
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DIAPH1 antibody (H00001729-B01) by Western Blots.
SDS-PAGE Gel
DIAPH1 transfected lysate
Western Blot
Lane 1: DIAPH1 transfected lysate ( 44.44 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DIAPH1
Entrez GeneID
1729GeneBank Accession#
BC007411Protein Accession#
AAH07411Gene Name
DIAPH1
Gene Alias
DFNA1, DIA1, DRF1, FLJ25265, LFHL1, hDIA1
Gene Description
diaphanous homolog 1 (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000195047|OTTHUMP00000195048|diaphanous 1|diaphanous-1|diaphanous-related formin 1
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Interactome
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Pathway
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Disease
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