NQO1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NQO1 full-length
Host
Human
Theoretical MW (kDa)
30.9
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NQO1 antibody (H00001728-B01) by Western Blots.
SDS-PAGE Gel
NQO1 transfected lysate.
Western Blot
Lane 1: NQO1 transfected lysate ( 30.9 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NQO1
Entrez GeneID
1728GeneBank Accession#
NM_000903.2Protein Accession#
-Gene Name
NQO1
Gene Alias
DHQU, DIA4, DTD, NMOR1, NMORI, QR1
Gene Description
NAD(P)H dehydrogenase, quinone 1
Omim ID
125860Gene Ontology
HyperlinkGene Summary
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
DT-diaphorase|NAD(P)H menadione oxidoreductase 1, dioxin-inducible|NAD(P)H:Quinone acceptor oxidoreductase type 1|NAD(P)H:menadione oxidoreductase 1|NAD(P)H:quinone oxireductase|azoreductase|diaphorase (NADH/NADPH) (cytochrome b-5 reductase)|diaphorase-4|
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Interactome
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Disease
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