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CYB5R3 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001727-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CYB5R3 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 34.2
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CYB5R3 antibody (H00001727-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001727-T01
    CYB5R3 transfected lysate.
    Western Blot
    QC Testing of H00001727-T01
    Lane 1: CYB5R3 transfected lysate ( 34.2 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1727
  • Protein Accession#:
  • -
  • Gene Name:
  • CYB5R3
  • Gene Alias:
  • B5R,DIA1
  • Gene Description:
  • cytochrome b5 reductase 3
  • Gene Summary:
  • This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq
  • Other Designations:
  • NADH-cytochrome b5 reductase,OTTHUMP00000028761,cytochrome b5 reductase,diaphorase (NADH) (cytochrome b-5 reductase)
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