DHPS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DHPS transfected lysate
Western Blot
Lane 1: DHPS transfected lysate ( 41 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DHPS full-length
Host
Human
Theoretical MW (kDa)
41
Interspecies Antigen Sequence
Mouse (91); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DHPS antibody (H00001725-B02) by Western Blots.
SDS-PAGE Gel
DHPS transfected lysate
Western Blot
Lane 1: DHPS transfected lysate ( 41 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DHPS
Entrez GeneID
1725GeneBank Accession#
NM_001930Protein Accession#
NP_001921Gene Name
DHPS
Gene Alias
MIG13
Gene Description
deoxyhypusine synthase
Omim ID
600944Gene Ontology
HyperlinkGene Summary
The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A. In the first step of hypusine biosynthesis, deoxyhypusine synthase catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. This gene consists of nine exons spanning 6.6 kb. Three transcript variants have been isolated. However, only transcript variant 1 encodes an active protein. The shorter variants may act as modulating factors of DHPS activity. [provided by RefSeq
Other Designations
migration-inducing gene 13
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