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DHPS 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001725-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-DHPS full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 41
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DHPS antibody (H00001725-B02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001725-T01
    DHPS transfected lysate
    Western Blot
    QC Testing of H00001725-T01
    Lane 1: DHPS transfected lysate ( 41 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1725
  • Gene Name:
  • DHPS
  • Gene Alias:
  • MIG13
  • Gene Description:
  • deoxyhypusine synthase
  • Gene Summary:
  • The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A. In the first step of hypusine biosynthesis, deoxyhypusine synthase catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. This gene consists of nine exons spanning 6.6 kb. Three transcript variants have been isolated. However, only transcript variant 1 encodes an active protein. The shorter variants may act as modulating factors of DHPS activity. [provided by RefSeq
  • Other Designations:
  • migration-inducing gene 13
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