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DHFR 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001719-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-DHFR full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 21.5
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DHFR antibody (H00001719-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001719-T01
    DHFR transfected lysate.
    Western Blot
    QC Testing of H00001719-T01
    Lane 1: DHFR transfected lysate ( 21.5 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1719
  • Protein Accession#:
  • -
  • Gene Name:
  • DHFR
  • Gene Alias:
  • -
  • Gene Description:
  • dihydrofolate reductase
  • Gene Summary:
  • Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq
  • Other Designations:
  • -
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