DHFR 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DHFR full-length
Host
Human
Theoretical MW (kDa)
21.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DHFR antibody (H00001719-B01) by Western Blots.
SDS-PAGE Gel
DHFR transfected lysate.
Western Blot
Lane 1: DHFR transfected lysate ( 21.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DHFR
Entrez GeneID
1719GeneBank Accession#
NM_000791.3Protein Accession#
-Gene Name
DHFR
Gene Alias
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Gene Description
dihydrofolate reductase
Omim ID
126060Gene Ontology
HyperlinkGene Summary
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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