COCH 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
COCH transfected lysate.
Western Blot
Lane 1: COCH transfected lysate ( 53.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-COCH full-length
Host
Human
Theoretical MW (kDa)
53.2
Interspecies Antigen Sequence
Mouse (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-COCH antibody (H00001690-B01) by Western Blots.
SDS-PAGE Gel
COCH transfected lysate.
Western Blot
Lane 1: COCH transfected lysate ( 53.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — COCH
Entrez GeneID
1690GeneBank Accession#
BC007230Protein Accession#
AAH07230Gene Name
COCH
Gene Alias
COCH-5B2, COCH5B2, DFNA9
Gene Description
coagulation factor C homolog, cochlin (Limulus polyphemus)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq
Other Designations
cochlin
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