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COCH 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001690-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-COCH full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 53.2
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-COCH antibody (H00001690-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001690-T01
    COCH transfected lysate.
    Western Blot
    QC Testing of H00001690-T01
    Lane 1: COCH transfected lysate ( 53.2 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1690
  • Gene Name:
  • COCH
  • Gene Alias:
  • COCH-5B2,COCH5B2,DFNA9
  • Gene Description:
  • coagulation factor C homolog, cochlin (Limulus polyphemus)
  • Gene Summary:
  • The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq
  • Other Designations:
  • cochlin
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