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DFFA 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001676-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-DFFA full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 36.5
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DFFA antibody (H00001676-D01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001676-T02
    DFFA transfected lysate.
    Western Blot
    QC Testing of H00001676-T02
    Lane 1: DFFA transfected lysate ( 36.5 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1676
  • Gene Name:
  • DFFA
  • Gene Alias:
  • DFF-45,DFF1,ICAD
  • Gene Description:
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • Gene Summary:
  • Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • DFF45,DNA fragmentation factor, 45 kD, alpha polypeptide,DNA fragmentation factor, 45 kD, alpha subunit,OTTHUMP00000001903,OTTHUMP00000001904
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