DFFA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DFFA transfected lysate.
Western Blot
Lane 1: DFFA transfected lysate ( 36.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DFFA full-length
Host
Human
Theoretical MW (kDa)
36.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DFFA antibody (H00001676-D01) by Western Blots.
SDS-PAGE Gel
DFFA transfected lysate.
Western Blot
Lane 1: DFFA transfected lysate ( 36.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DFFA
Entrez GeneID
1676GeneBank Accession#
NM_004401Protein Accession#
NP_004392.1Gene Name
DFFA
Gene Alias
DFF-45, DFF1, ICAD
Gene Description
DNA fragmentation factor, 45kDa, alpha polypeptide
Omim ID
601882Gene Ontology
HyperlinkGene Summary
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
DFF45|DNA fragmentation factor, 45 kD, alpha polypeptide|DNA fragmentation factor, 45 kD, alpha subunit|OTTHUMP00000001903|OTTHUMP00000001904
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Interactome
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Pathway
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Disease
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