This antibody pair set comes with matched antibody pair to detect and quantify protein level of human DFFA.
Quality Control Testing:
Standard curve using recombinant protein ( H00001676-P01 ) as an analyte.
Sandwich ELISA detection sensitivity ranging from 0.3 ng/ml to 100 ng/ml.
Antibody pair set content: 1. Capture antibody: rabbit MaxPab® affinity purified polyclonal anti-DFFA (100 ug) 2. Detection antibody: mouse monoclonal anti-DFFA, IgG2a Kappa (20 ug) *Reagents are sufficient for at least 1-2 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq