CFD (Human) Recombinant Protein
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Specification
Product Description
Purified CFD (NP_001919.2 26 a.a. - 253 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
Transfected Cell Line
Human HEK293H cells
Sequence
ILGGREAEAHARPYMASVQLNGAHLCGGVLVAEQWVLSAAHCLEDAADGKVQVLLGAHSLSQPEPSKRLYDVLRAVPHPDSQPDTIDHDLLLLQLSEKATLGPAVRPLPWQRVDRDVAPGTLCDVAGWGIVNHAGRRPDSLQHVLLPVLDRATCNRRTHHDGAITERLMCAESNRRDSCKGDSGGPLVCGGVLEGVVTSGSRVCGNRKKPGIYTRVASYAAWIDSVLA
Host
Human
Theoretical MW (kDa)
28.16
Form
Liquid
Preparation Method
Transfection of pSuper-CFD plasmid into HEK293H cell, and the expressed protein was purified by Strep-Tactin affinity column.
Purification
Strep-Tactin affinity columns
Concentration
>= 10 ug/ml
Quality Control Testing
SDS-PAGE and Western Blot
SDS-PAGE Gel
Western Blot
Storage Buffer
100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
Protein Interaction
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Gene Info — CFD
Entrez GeneID
1675GeneBank Accession#
NM_001928.2Protein Accession#
NP_001919.2Gene Name
CFD
Gene Alias
ADIPSIN, ADN, DF, PFD
Gene Description
complement factor D (adipsin)
Omim ID
134350Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. [provided by RefSeq
Other Designations
C3 convertase activator|D component of complement (adipsin)|complement factor D|complement factor D preproprotein|properdin factor D
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Interactome
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Pathway
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Disease
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