DEFA5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DEFA5 full-length
Host
Human
Theoretical MW (kDa)
10.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DEFA5 antibody (H00001670-D01P) by Western Blots.
SDS-PAGE Gel
DEFA5 transfected lysate.
Western Blot
Lane 1: DEFA5 transfected lysate ( 10.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DEFA5
Entrez GeneID
1670GeneBank Accession#
NM_021010.1Protein Accession#
NP_066290.1Gene Name
DEFA5
Gene Alias
DEF5, HD-5, MGC129728
Gene Description
defensin, alpha 5, Paneth cell-specific
Omim ID
600472Gene Ontology
HyperlinkGene Summary
Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq
Other Designations
defensin 5|defensin, alpha 5|defensin, alpha 5, preproprotein
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