DDX11 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
DDX11 transfected lysate.
Western Blot
Lane 1: DDX11 transfected lysate ( 106.81 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DDX11 full-length
Host
Human
Theoretical MW (kDa)
106.81
Interspecies Antigen Sequence
Mouse (76)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDX11 antibody (H00001663-B01) by Western Blots.
SDS-PAGE Gel
DDX11 transfected lysate.
Western Blot
Lane 1: DDX11 transfected lysate ( 106.81 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DDX11
Entrez GeneID
1663GeneBank Accession#
BC050522Protein Accession#
AAH50522Gene Name
DDX11
Gene Alias
CHL1, CHLR1, KRG2, MGC133249, MGC9335
Gene Description
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae)
Omim ID
601150Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
CHL1-related helicase gene-1|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11|keratinocyte growth factor-regulated gene 2
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