DDX6 monoclonal antibody (M01), clone 3D2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant DDX6.
Immunogen
DDX6 (AAH65007, 1 a.a. ~ 483 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (78.87 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
DDX6 monoclonal antibody (M01), clone 3D2 Western Blot analysis of DDX6 expression in Hela ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of DDX6 expression in transfected 293T cell line by DDX6 monoclonal antibody (M01), clone 3D2.
Lane 1: DDX6 transfected lysate(53.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to DDX6 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of DDX6 over-expressed 293 cell line, cotransfected with DDX6 Validated Chimera RNAi ( Cat # H00001656-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DDX6 monoclonal antibody (M01), clone 3D2 (Cat # H00001656-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — DDX6
Entrez GeneID
1656GeneBank Accession#
BC065007Protein Accession#
AAH65007Gene Name
DDX6
Gene Alias
FLJ36338, HLR2, P54, RCK
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Omim ID
600326Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. [provided by RefSeq
Other Designations
DEAD box-6|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
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Interactome
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Pathway
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Publication Reference
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Cytoplasmic aggregation of DDX1 in developing embryos: Early embryonic lethality associated with Ddx1 knockout.
Hildebrandt MR, Wang Y, Li L, Yasmin L, Glubrecht DD, Godbout R.
Developmental Biology 2019 Jul; [Epub].
Application:IF, Mouse, Mouse embryos.
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DDX6 localizes to nuage structures and the annulus of mammalian spermatogenic cells.
Kawahara C, Yokota S, Fujita H.
Histochemistry and Cell Biology 2014 Jan; 141(1):111.
Application:IF, WB-Ti, IEM, Rat, Mouse, Testis.
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The DEAD-box RNA Helicase DDX6 is Required for Efficient Encapsidation of a Retroviral Genome.
Yu SF, Lujan P, Jackson DL, Emerman M, Linial ML.
PLoS Pathogens 2011 Oct; 7(10):e1002303.
Application:IF, WB, Human, HT1080, 293T cells.
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Rck/p54 interacts with APP mRNA as part of a multi-protein complex and enhances APP mRNA and protein expression in neuronal cell lines.
Broytman O, Westmark PR, Gurel Z, Malter JS.
Neurobiology of Aging 2008 Apr; 30(12):1962.
Application:WB, Human, SH-SY5Y cells.
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Cytoplasmic aggregation of DDX1 in developing embryos: Early embryonic lethality associated with Ddx1 knockout.
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