DDX5 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DDX5 full-length
Host
Human
Theoretical MW (kDa)
67.65
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDX5 antibody (H00001655-B01) by Western Blots.
Western Blot
Lane 1: DDX5 transfected lysate ( 67.54 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
DDX5 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DDX5
Entrez GeneID
1655GeneBank Accession#
BC016027Protein Accession#
AAH16027Gene Name
DDX5
Gene Alias
DKFZp434E109, DKFZp686J01190, G17P1, HLR1, HUMP68, p68
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Omim ID
180630Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX5|DEAD box-5|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)
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Interactome
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Disease
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