AKR1C2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
AKR1C2 transfected lysate.
Western Blot
Lane 1: AKR1C2 transfected lysate ( 36.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AKR1C2 full-length
Host
Human
Theoretical MW (kDa)
36.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AKR1C2 antibody (H00001646-D01P) by Western Blots.
SDS-PAGE Gel
AKR1C2 transfected lysate.
Western Blot
Lane 1: AKR1C2 transfected lysate ( 36.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AKR1C2
Entrez GeneID
1646GeneBank Accession#
NM_001354.4Protein Accession#
AAH07024.1Gene Name
AKR1C2
Gene Alias
AKR1C-pseudo, BABP, DD, DD2, DDH2, HAKRD, HBAB, MCDR2
Gene Description
aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III)
Omim ID
600450Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq
Other Designations
OTTHUMP00000018995|OTTHUMP00000044759|aldo-keto reductase family 1, member C2|chlordecone reductase homolog|pseudo-chlordecone reductase|trans-1,2-dihydrobenzene-1,2-diol dehydrogenase|type II dihydrodiol dehydrogenase
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Interactome
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Pathway
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Disease
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