DCI 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DCI transfected lysate.
Western Blot
Lane 1: DCI transfected lysate ( 26.84 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DCI full-length
Host
Human
Theoretical MW (kDa)
26.84
Interspecies Antigen Sequence
Mouse (76); Rat (79)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DCI antibody (H00001632-B01) by Western Blots.
SDS-PAGE Gel
DCI transfected lysate.
Western Blot
Lane 1: DCI transfected lysate ( 26.84 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DCI
Entrez GeneID
1632GeneBank Accession#
BC009631.1Protein Accession#
AAH09631.1Gene Name
DCI
Gene Alias
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Gene Description
dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
Omim ID
600305Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq
Other Designations
3,2 trans-enoyl-Coenzyme A isomerase|D3,D2-enoyl-CoA isomerase|acetylene-allene isomerase|delta3, delta2-enoyl-CoA isomerase|dodecenoyl-CoA delta Isomerase|dodecenoyl-Coenzyme A delta isomerase
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Interactome
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Pathway
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