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DBT 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001629-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-DBT full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 53.13
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DBT antibody (H00001629-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001629-T01
    DBT transfected lysate.
    Western Blot
    QC Testing of H00001629-T01
    Lane 1: DBT transfected lysate ( 53.13 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1629
  • Gene Name:
  • DBT
  • Gene Alias:
  • BCATE2,E2,E2B,MGC9061
  • Gene Description:
  • dihydrolipoamide branched chain transacylase E2
  • Gene Summary:
  • The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq
  • Other Designations:
  • BCKAD E2 subunit,E2 component of branched chain alpha-keto acid dehydrogenase complex,OTTHUMP00000012596,branched chain acyltransferase, E2 component,dihydrolipoamide branched chain transacylase,dihydrolipoyl transacylase,dihydrolipoyllysine-residue (2-me
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