DBT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DBT transfected lysate.
Western Blot
Lane 1: DBT transfected lysate ( 53.13 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DBT full-length
Host
Human
Theoretical MW (kDa)
53.13
Interspecies Antigen Sequence
Mouse (88); Rat (88)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DBT antibody (H00001629-B01) by Western Blots.
SDS-PAGE Gel
DBT transfected lysate.
Western Blot
Lane 1: DBT transfected lysate ( 53.13 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DBT
Entrez GeneID
1629GeneBank Accession#
BC016675.1Protein Accession#
AAH16675.1Gene Name
DBT
Gene Alias
BCATE2, E2, E2B, MGC9061
Gene Description
dihydrolipoamide branched chain transacylase E2
Gene Ontology
HyperlinkGene Summary
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq
Other Designations
BCKAD E2 subunit|E2 component of branched chain alpha-keto acid dehydrogenase complex|OTTHUMP00000012596|branched chain acyltransferase, E2 component|dihydrolipoamide branched chain transacylase|dihydrolipoyl transacylase|dihydrolipoyllysine-residue (2-me
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Interactome
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Pathway
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