DBI 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DBI transfected lysate.
Western Blot
Lane 1: DBI transfected lysate ( 11.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DBI full-length
Host
Human
Theoretical MW (kDa)
11.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DBI antibody (H00001622-D01P) by Western Blots.
SDS-PAGE Gel
DBI transfected lysate.
Western Blot
Lane 1: DBI transfected lysate ( 11.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DBI
Entrez GeneID
1622GeneBank Accession#
NM_020548Protein Accession#
NP_065438.1Gene Name
DBI
Gene Alias
ACBD1, ACBP, CCK-RP, EP, MGC70414
Gene Description
diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein)
Omim ID
125950Gene Ontology
HyperlinkGene Summary
This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
GABA receptor modulator|acyl coenzyme A binding protein|acyl-Coenzyme A binding domain containing 1|cholecystokinin-releasing peptide, trypsin-sensitive|diazepam binding inhibitor|diazepam binding inhibitor, splice form 1c|endozepine
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Interactome
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Pathway
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Disease
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