DGKG 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DGKG transfected lysate.
Western Blot
Lane 1: DGKG transfected lysate ( 84.37 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DGKG full-length
Host
Human
Theoretical MW (kDa)
84.37
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DGKG antibody (H00001608-B01) by Western Blots.
SDS-PAGE Gel
DGKG transfected lysate.
Western Blot
Lane 1: DGKG transfected lysate ( 84.37 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DGKG
Entrez GeneID
1608GeneBank Accession#
BC089411Protein Accession#
AAH89411Gene Name
DGKG
Gene Alias
DAGK3, DGK-GAMMA, MGC104993, MGC133330
Gene Description
diacylglycerol kinase, gamma 90kDa
Omim ID
601854Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
DAG kinase gamma|diacylglycerol kinase gamma|diacylglyerol kinase gamma|diglyceride kinase gamma
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Interactome
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Pathway
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Disease
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