CYP27A1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CYP27A1 full-length
Host
Human
Theoretical MW (kDa)
60.2
Interspecies Antigen Sequence
Mouse (72); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CYP27A1 antibody (H00001593-B01) by Western Blots.
SDS-PAGE Gel
CYP27A1 transfected lysate.
Western Blot
Lane 1: CYP27A1 transfected lysate ( 60.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CYP27A1
Entrez GeneID
1593GeneBank Accession#
NM_000784.2Protein Accession#
-Gene Name
CYP27A1
Gene Alias
CP27, CTX, CYP27
Gene Description
cytochrome P450, family 27, subfamily A, polypeptide 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq
Other Designations
5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase|5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase|cholestanetriol 26-monooxygenase|cytochrome P-450C27/25|cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrot
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Interactome
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Pathway
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Disease
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