CYP27A1 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of CYP27A1 transfected lysate using rabbit polyclonal anti-CYP27A1 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-CYP27A1.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (71%); Rat (72%)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of CYP27A1 transfected lysate using rabbit polyclonal anti-CYP27A1 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-CYP27A1.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-CYP27A1 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-CYP27A1 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — CYP27A1
Entrez GeneID
1593Gene Name
CYP27A1
Gene Alias
CP27, CTX, CYP27
Gene Description
cytochrome P450, family 27, subfamily A, polypeptide 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq
Other Designations
5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase|5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase|cholestanetriol 26-monooxygenase|cytochrome P-450C27/25|cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrot
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Interactome
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Pathway
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Disease
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